| LAL Technical Data Sheet
Limulus Amebocyte Lysate (LAL) Kinetic Chromogenic Assay Tests were performed at an independent laboratory on ErtelAlsop filter media to determine the concentration of endotoxin units per ml extracted from the filter media. Procedure: A 12.0 gram portion was covered with 240 ml of sterile nonpyrogenic water for injection and were extracted for 40 to 60 minutes at 37° C to 40° C in a shaker incubator. One-tenth ml of the test and control solutions was placed in a sterile microplate well and incubated at 37± 1° C for ten minutes in the Kinetic-Chromogenic Reader. One-tenth ml of Lysate, reconstituted per manufacturer’s current directions, was then added to each well and testing was engaged. The quantity of endotoxin, determined spectrophotometrically, and its reaction time, with the LAL/Substrate, was compared to that of a standard curve to determine endotoxin concentration. Results:
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